Development of rabbit monoclonal antibodies for detection of alpha-dystroglycan in normal and dystrophic tissue.
Alpha-dystroglycan requires a rare O-mannose glycan modification to form its binding epitope for extracellular matrix proteins such as laminin.This functional glycan is disrupted in a cohort of muscular dystrophies, the secondary dystroglycanopathies, and is abnormal in some metastatic cancers.The most commonly used reagent for detection of alpha-d